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Objective To investigate the comparison of rubber gloves collection bag, linear closing type plastic collection bags and plastic specimen collection bag is used for application effect of laparoscopic ovarian cyst resection. Methods From 2013 June to 2014 December underwent laparoscopic ovarian cyst resection in 90 cases, according to the first date of surgery were divided into 3 groups, from the standard sample collection bag for rubber gloves (A group) 30 cases, linear closing type plastic collection bag (B group) 30 cases, collecting bag plastic specimens (C group) 30 cases. Respectively calculate autologous peritoneal bag mouth opening into time, time to complete transection of tissue into the specimen collection bag time, specimen collection bag left abdominal contents to the in vitro observation time, specimen collection bags in use in the course of rupture occurrence rate of specimen content leakage. Results The 3 groups of patients with autologous abdominal time were (7.18 ± 0.61),(6.72 ± 0.57), (5.98 ± 0.30) s, the difference was not statistically significant (t=41.80, P>0.01). The 3 groups of specimens collected pouch specimens respectively (40.84± 6.27), (49.86 ± 6.10), (32.98 ± 3.93) s, group C open pocket to maintain state of distraction to amputated tissue fully into the specimen collection bag in the shortest time, with a group and B group, the difference was statistically significant (F=69.87, P<0.01). The 3 groups of specimens collected from the abdominal cavity were (498.39±55.60), (315.70±47.32), (448.65±54.90) s, the difference was statistically significant (F=96.18, P < 0.01). The 3 groups of specimens collected specimen bag collection bag rupture were 26.7% (8/ 30),10.0% (3/ 30),3.33% (1/ 30), group A of the most common (χ2=0.926, P < 0.05), The 3 groups of specimens collected the bag contents leakage were 23.30%(7/30), 6.67%(2/30)、3.33%(1/30), group A of frequency was the highest, and in group B and C, the difference had statistical significance (χ2=0.635, P<0.05). Conclusions Plastic type specimen collection bag for soft bag with built-in wire, the bag opening is a certain tension, easy operation by operation control, convenient and reliable safety, shorten operation time, reduce the occurrence of complications, reduce surgical risks and costs, and improve the office efficiency and quality of the operation.
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Objective:To investigate the effect of DNA vaccine expressed Helicobacter pylori(Hp) oipA on protecting against Hp infection.Methods:The ORFs of Hp oipA had been inserted into the eukaryotic expressing vector pVAX1 and SGC-7901 cells had been transfected with recombinant plasmid pVAX1-oipA. The expression of oipA had been detected by RT-PCR and ELISA. After extracted and purified, pVAX1-oipA had been injected into BALB/c mice through muscles of right leg one time each week for three weeks(100 ?g each mouse). pVAX1 blank and normal saline had been used as the control groups. Titer of antibodies had been detected by ELISA two months after the last immunization. Based on the confirmation of immunological response in the pVAX1 groups, mice had been given orogastric challenged with live Hp Sydney strain three times(0.5?108/0.5 ml each mouse). Four weeks after challenge, mice had been sacrificed. Histological change and the colonization of Hp in the gastric mucosa had been detected by urease test, the culture of Hp, and electronic microscopy.Results:SGC-7901 cells transfected with pVAX1-oipA had expressed corresponding production at the level of transcription and translation. Immunized mice had been induced anti-oipA antibodies. After challenged with bacterium, as contrast to immunized mice groups injected with pVAX1-oipA, pVAX1 blank, normal saline, the positive rate of urease test of gastric mucosa was 0(0/10), 90%(9/10), 100%(5/5)respectively,the positive rate of cultures of Hp was 20%(2/10), 90%(9/10), 100%(5/5)respectively. Histological findings: the different degree of erosion had been observed in control group, but 80%(8/10)of gastric mucosa were normal in immunized mice.Conclusion:oipA DNA could induce effective immune response in protection against Hp infection.
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0.05) between the apoptosis rate of SpcDNA3.1 and that of SoipA,SureC.Conclusion:Expression product of UreC and OipA that have antigenicity had no effect on cell function, so they could be prepared for DNA vaccine.